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Platelet and leukocyte activation during aortoiliac angiography and angioplasty
J Barani1, A Gottsäter, I Mattiasson
1Department of Vascular Diseases, University of Lund, University Hospital, S-205 02 Malmö, Sweden.
Insights
Peripheral angiography and angioplasty activate platelets, indicated by changes in P-selectin expression and intraplatelet signaling molecules. Leukocyte activation was not observed during these procedures.
Area of Science:
- Cardiovascular Medicine
- Hematology
- Interventional Radiology
Background:
- Aortoiliac atherosclerotic disease poses significant risks.
- Angiography and percutaneous transluminal angioplasty (PTA) are common interventions.
- Understanding cellular activation during these procedures is crucial.
Purpose of the Study:
- To investigate platelet and leukocyte activation during aortoiliac angiography and PTA.
- To assess changes in specific platelet and granulocyte markers.
Main Methods:
- Observational study of 14 patients with aortoiliac atherosclerotic disease.
- Nine patients underwent PTA.
- Measured platelet and granulocyte activation markers in arterial and venous blood.
Main Results:
- Increased intraplatelet cAMP proximal to lesions before PTA.
- Increased intraplatelet cGMP proximal to lesions after PTA.
- Decreased P-selectin positive platelets and expression post-angiography; lower granulocyte CD18 expression.
Conclusions:
- Findings suggest platelet activation occurs during peripheral angiography.
- Leukocyte activation was not evident during the procedures.
- Further research may explore therapeutic interventions targeting platelet activation.
Objective:
to evaluate platelet and leukocyte activation during aortoiliac angiography and percutaneous transluminal angioplasty (PTA).
Methods:
an observational study of 14 patients with aortoiliac atherosclerotic disease, nine of whom underwent PTA. The proportion of fibrinogen-, and P-selectin positive platelets, P-selectin expression on platelets, intraplatelet cGMP and cAMP, CD18 positive granulocytes, CD18 expression on granulocytes, plasma (p)-neopterin, p-TNF alpha and p- interleukin-6 were repeatedly measured in arterial blood during angiography and in venous blood before and after.
Results:
compared to a previous venous sample, arterial intraplatelet cAMP was increased proximal to the atherosclerotic lesion before contrast infusion and PTA (median 18 [range: 14-22] vs 16 [15-21] pmol/10(9) platelets p<0.05), and intraplatelet cGMP was increased proximal to the lesion after contrast infusion and PTA (1.2 [0.8-3.9] vs 0.9 [0.6-2.5] pmol/10(9) platelets p<0.05). Four hours after angiography, both the proportion of P-selectin positive platelets (28[11-55]%) and platelet P-selectin expression (9[6-40]) had decreased (p<0.05), from arterial values distal to the lesion before contrast infusion and PTA (57 [24-78]% and 26 [10-83]). Granulocyte CD18 expression was lower during angiography than in a previous venous sample.
Conclusions:
the results are compatible with platelet but not leukocyte activation during peripheral angiography.