Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plasminogen has a broad extrahepatic distribution.

Lu Zhang1, Dietmar Seiffert, Bruce J Fowler

  • 1The Scripps Research Institute, Department of Vascular Biology, La Jolla, CA 92037, USA.

Thrombosis and Haemostasis
|March 28, 2002
PubMed
Summary

The plasminogen gene is widely expressed in many mouse tissues beyond the liver. This suggests that various organs can locally produce plasminogen, impacting tissue-specific functions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plg-R<sub>KT</sub> facilitates plasminogen incorporation and restrains thrombus growth under arterial shear in mice.

Molecular medicine (Cambridge, Mass.)·2026
Same author

Plasmin modulates neutrophilic inflammation and alveolar macrophage function, protecting mice from pneumococcal pneumonia.

Blood·2026
Same author

Macrophage Plg-R<sub>KT</sub> expression promotes diet-induced obesity and metabolic dysfunction-associated steatotic liver disease.

Journal of thrombosis and haemostasis : JTH·2025
Same author

Plasminogen and plasmin induce specialized proresolving mediators and promote efferocytosis via 5-lipoxygenase.

Journal of thrombosis and haemostasis : JTH·2025
Same author

Proteome-Wide Genetic Investigation of Large Artery Stiffness.

JACC. Basic to translational science·2024
Same author

Proteomic Correlates and Prognostic Significance of Kidney Injury in Heart Failure With Preserved Ejection Fraction.

Journal of the American Heart Association·2024

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Physiology

Background:

  • Plasmin, a key fibrinolytic enzyme, is primarily sourced from liver-produced plasminogen.
  • Plasmin's broad substrate range suggests potential roles beyond vascular fibrinolysis.
  • Investigating extrahepatic plasminogen expression is crucial for understanding tissue-specific functions.

Purpose of the Study:

  • To determine if plasminogen is expressed in tissues other than the liver.
  • To identify specific extrahepatic tissues and cell types expressing plasminogen mRNA.
  • To assess the potential for local plasminogen production in various organs.

Main Methods:

  • Development of a sensitive and specific isotopic quantitative RT-PCR assay for plasminogen mRNA detection.

Related Experiment Videos

  • Isolation of total RNA from various C57BL/6J mouse tissues.
  • Application of in situ hybridization to localize plasminogen mRNA expression within tissues.

    • Main Results:

    • Plasminogen mRNA was detected in multiple extrahepatic tissues, including adrenal, kidney, brain, testis, heart, lung, uterus, spleen, thymus, and gut.
    • Adrenal tissue exhibited the highest levels of plasminogen mRNA.
    • In situ hybridization confirmed plasminogen mRNA expression in adrenal and kidney cortexes and medullae, specific brain neurons, spermatogenesis cells, thymus, and spleen.

    Conclusions:

    • The plasminogen gene is broadly expressed across numerous extrahepatic tissues in mice.
    • These findings indicate that various tissues possess the capacity for local plasminogen synthesis.
    • Local plasminogen production may contribute to tissue-specific functions, independent of or in addition to circulating plasminogen.