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Identification and characterization of a gene encoding drought-inducible protein localizing in the bundle sheath cell
Bambang Sugiharto1, Netty Ermawati, Hitoshi Mori
1Research Center for Molecular Biology, University of Jember, Jl. Kalimantan III/23, Jember, 68121, Indonesia. sugiharto@binanusa.net
Abstract:
We have identified a drought-inducible gene, designated as SoDip22, in sugarcane leaves. The cDNA encoded a hydrophilic protein with a calculated molecular mass of 15.9 kDa and the amino acid sequence was similar to that of ABA, stress and ripening-inducible protein from various plant species. ABA or mannitol-treatment of the detached leaves also induced SoDip22 expression. Stepwise homogenization of the stressed leaves showed that SoDip22 is localized in bundle sheath cells. These results suggest that SoDip22 functions to adapt to drought stress in the bundle sheath cell, and that the signaling pathway for the induction is, at least in a part, mediated by ABA.