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Updated: Jul 31, 2026
Measurement of γHV68 Infection in Mice
Published on: November 22, 2011
Interleukin-6 stimulates HHV-8 replication in bone marrow cultures and infected cell lines
F Agbalika1, J P Marolleau, J C Brouet
1Laboratory of Virology, Hôpital Saint-Louis, Paris, France.
Introduction:
The significance of HHV-8 DNA detection in bone marrow stromal cells from patients with multiple myeloma is still controversial. Since IL-6 plays a key role in the pathogenesis of myeloma, we studied the effect of this lymphokine on HHV-8 DNA detection.
Materials And Methods:
Amplification of HHV-8 DNA from long-term bone marrow cultures established from normal individuals in the presence or absence of 1 ng/ml IL-6 and from an HHV-8 infected ISI cell line.
Results And Conclusions:
IL-6 increased HHV-8 replication in seven of ten bone marrow cultures as well as in the ISI cell line. Quantitative PCR showed a 3-100-fold increase in HHV-8 DNA copy number/microg DNA. These data suggest that when IL-6 is present in the micro-environment, HHV-8 replicates and may be amplified in the absence of systemic infection in patients without cellular immune deficiency.
