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Updated: Jul 29, 2026
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An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells
Published on: July 15, 2015
Cyclin D1 expression in patients with multiple myeloma.
X Troussard1, H Avet-Loiseau, M Macro
1Laboratoire d'Hématologie, CHU Caen, France, and UPRES-EA 2128, UFR de Médecine, Caen, France. troussard-x@chu-caen.fr
Summary
Cyclin D1 expression occurs in 50% of multiple myeloma (MM) patients. This expression is linked to chromosomal abnormalities, specifically t(11;14) or extra chromosome 11 copies.
Area of Science:
- Hematology
- Oncology
- Genetics
Background:
- Chromosomal abnormalities are prevalent in multiple myeloma (MM), affecting 50-70% of patients.
- The t(11;14)(q13;q32) translocation is found in 3-14% of MM cases via conventional cytogenetics.
Purpose of the Study:
- To investigate cyclin D1 expression in multiple myeloma (MM) patients and cell lines.
- To determine the association between cyclin D1 expression and chromosomal abnormalities, particularly t(11;14).
Main Methods:
- Screening of 14 MM patients and nine human multiple myeloma cell lines (HMCL) for cyclin D1 expression using competitive RT-PCR and/or Northern blot.
- Fluorescence in situ hybridization (FISH) analysis of 10 MM patients to detect t(11;14) and chromosome 11 copy number variations.
Main Results:
- Cyclin D1 overexpression was observed in five HMCL, with three showing t(11;14) and two having extra chromosome 11 copies.
- Cyclin D1 expression was detected in 50% (7/14) of untreated MM patients at diagnosis.
- FISH analysis revealed t(11;14) in 30% (3/10) and extra chromosome 11 copies in 20% (2/10) of the studied MM patients.
Conclusions:
- Cyclin D1 expression is a frequent event in multiple myeloma (50% of patients).
- This expression is associated with either the t(11;14)(q13;q32) translocation or extra copies of chromosome 11.
- The precise prognostic significance of cyclin D1 expression in MM requires further investigation, especially in comparison to other B-cell lymphoproliferative disorders.
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