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Related Experiment Videos

Ecto-diadenosine polyphosphates hydrolase activity on human prostasomes.

Alba Minelli1, Cinzia Allegrucci, Lavinia Liguori

  • 1Dipartimento di Scienze Biochimiche e Biotecnologie Molecolari, Sezione di Biochimica Cellulare, Università di Perugia, Via del Giochetto, Perugia, Italy. albami@tin.it

The Prostate
|March 29, 2002
PubMed
Summary

Human prostasomes possess an ecto-diadenosine polyphosphates hydrolase that degrades diadenosine compounds. This enzyme can transfer to spermatozoa, suggesting a novel role for prostasomes in fertilization.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Reproductive Biology

Background:

  • Ecto-diadenosine polyphosphates are signaling molecules with diverse physiological functions.
  • Ecto-diadenosine polyphosphates hydrolase enzymes regulate these signals by degradation.
  • Human prostasomes are investigated for the presence and function of this hydrolase.

Purpose of the Study:

  • To identify and partially characterize ecto-diadenosine polyphosphates hydrolase in human prostasomes.
  • To understand the substrate specificity and kinetic properties of the prostasomal enzyme.
  • To explore the enzyme's localization, activation, and potential transfer to spermatozoa.

Main Methods:

  • Utilized reverse-phase and paired-ion High-Performance Liquid Chromatography (HPLC) techniques.

Related Experiment Videos

  • Investigated enzyme kinetics and substrate preferences.
  • Examined enzyme properties including membrane association, cofactor activation, and pH optimum.

    • Main Results:

    • Human prostasomes possess an ecto-diadenosine polyphosphates hydrolase that degrades various diadenosine compounds.
    • Diadenosine tetraphosphate is the preferred substrate, further metabolized to adenosine.
    • The enzyme is membrane-bound via a GPI-anchor, activated by divalent cations (Ca+2, Mg+2, Mn+2), and functions optimally at a slightly alkaline pH.
    • Spermatozoa acquire this hydrolytic activity upon fusion with prostasomes.

    Conclusions:

    • The presence of a transferable diadenosine-degrading enzyme on prostasomes indicates a significant role in fertilization.
    • Prostasomes may modulate sperm function through the enzymatic transfer of ecto-diadenosine polyphosphates hydrolase.
    • This finding opens new avenues for understanding male fertility and reproductive processes.