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Updated: Oct 2, 2026
The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions
Published on: December 15, 2012
Tools for the study of genome rearrangements in laboratory and industrial yeast strains
Lesley Lockhart1, Stephen G Oliver, Daniela Delneri
1School of Biological Sciences, University of Manchester, 2.205 Stopford Building, Oxford Road, Manchester M13 9PT, UK.
Abstract:
In order to investigate the extent of genome rearrangements in laboratory and industrial yeast strains, a set of plasmids, containing ca. 300 bp fragments from highly conserved genes from S. cerevisiae, has been constructed. We chose three unique PCR products, each from a single gene, per chromosome: one from close to the centromere, and one from each chromosome end. Using these plasmids as probes to hybridize a Southern blot from a pulsed-field gel electrophoresis separation of the 16 yeast chromosomes, it is possible to identify large chromosomal rearrangements such as reciprocal translocations.
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