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A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication
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Metabolic co-operation between biochemically marked mammalian cells in tissue culture. 1969

H Subak-Sharpe1, R R Bürk, J D Pitts

  • 1Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.

Reviews in Medical Virology
|March 29, 2002
PubMed
Abstract

No abstract available in PubMed .

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A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...