Distribution of exogenous heavy metals in the hepatocytes of calves: a morphometric study

The objective of this study was to evaluate the presence of heavy metals in the hepatocytes of the animals fed a cadmium-supplemented diet and also receiving zinc and/or selenium in the injection form. The experiment involved four groups of calves (6-8, both sexes) receiving the heavy metals in various combinations for 95 days. Electron micrographs of liver cells were prepared and statistically evaluated using Student's t-test. A modified morphometric apparatus was used for morphometric examination. Exogenous cadmium showed marked accumulation in the hepatocytes. If, however, the cadmium diet was combined with zinc or selenium administration the amount of the reduction product was much lower.