Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Functional proteomics using chromophore-assisted laser inactivation.

Susanne Rubenwolf1, Jens Niewöhner, Elisabeth Meyer

  • 1Xerion-Pharmaceuticals, Martinsried/Munich, Germany. s.rubenwolf@xerion-pharma.com

Proteomics
|March 29, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of ASO conjugation and receptor binding affinity on intracellular transport of mono- and bispecific TfR- and CD98-Brainshuttle<sup>TM</sup> variants.

mAbs·2026
Same author

Three decades shaping infection prevention and control: a narrative review on Petra Gastmeier's achievements.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2026
Same author

A Reference-Free Algorithm Discovers Regulation in the Plant Transcriptome.

Plant direct·2026
Same author

2025 White Paper on Recent Issues in Bioanalysis: Biomarkers Calibrators & Stability; Evaluation of NULISA; Neurofilament & Autoantibody Biomarker Assays; Removing IgM Interference; ELISpot & FluoroSpot Best Practices; Modular HD Cytometry; Single-cell Analysis Imaging Cytometry (PART 2A - Recommendations on Biomarkers Discovery, Development, Validation & Regulatory Approval, Ligand-Binding Assays (LBA) and Cell-Based Assays (CBA) PART 2B - Regulatory Agencies' Input on Biomarkers, IVD/CDx and Biomarker Assay Validation (BAV)).

Bioanalysis·2026
Same author

Refined ADA testing strategy to overcome sRANKL interference in denosumab/FKS518 immunogenicity assessment.

Bioanalysis·2025
Same author

Development and validation of a method for quantitation of CTx-1 for the assessment of biosimilarity of FKS518, a denosumab biosimilar.

Bioanalysis·2025

Chromophore-assisted laser inactivation (CALI) precisely targets and inactivates specific proteins, crucial for drug discovery. This versatile method validates disease-relevant protein targets with over 50% inactivation achieved in various assays.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Background:

  • Proteins are key cellular components and major drug targets.
  • Chromophore-assisted laser inactivation (CALI) offers localized and timely protein functional disruption.
  • CALI utilizes dye-coupled ligands and laser irradiation to generate radicals for targeted covalent modification.

Purpose of the Study:

  • To demonstrate the efficacy of CALI for specific protein target inactivation.
  • To validate CALI as a versatile tool for drug target identification and validation.
  • To develop scalable assays for quantifying CALI-induced protein inactivation.

Main Methods:

  • Application of CALI using dye-coupled ligands and laser irradiation.
  • Development of high-throughput screening-scaleable assays for inactivation quantification.

Related Experiment Videos

  • Testing CALI in cell-free and cell-based assays, including human primary and tumor-derived cell lines.

    • Main Results:

    • Specific inactivation of multiple protein targets within signal transduction pathways.
    • Achieved at least 50% inactivation for all tested protein targets.
    • Demonstrated CALI's effectiveness in both cell-free and cell-based experimental systems.

    Conclusions:

    • CALI is a highly versatile and effective tool for validating disease-relevant protein targets.
    • The method accounts for post-translational modifications, broadening its applicability.
    • CALI facilitates precise functional assessment of proteins crucial for therapeutic development.