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Methods of analysis for ochratoxin A.

Peter M Scott1

  • 1Health Canada, Ottawa, Ontario.

Advances in Experimental Medicine and Biology
|April 2, 2002
PubMed
Summary
This summary is machine-generated.

Accurate detection of the harmful mycotoxin ochratoxin A (OTA) in food is crucial. Sensitive methods like immunoaffinity chromatography and liquid chromatography are vital for monitoring OTA levels and ensuring food safety.

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Area of Science:

  • Food chemistry
  • Analytical chemistry
  • Toxicology

Background:

  • Mycotoxin ochratoxin A (OTA) is produced by Aspergillus and Penicillium fungi.
  • OTA exhibits carcinogenic, nephrotoxic, teratogenic, and immunosuppressive properties.
  • Regulatory limits for OTA in foodstuffs necessitate sensitive determination methods.

Purpose of the Study:

  • To review methods for the extraction, cleanup, and determination of ochratoxin A in foodstuffs.
  • To highlight the importance of sensitive and reliable analytical techniques for OTA detection.

Main Methods:

  • Extraction using organic solvents with acid or aqueous sodium bicarbonate.
  • Cleanup via aqueous sodium bicarbonate partitioning, solid phase extraction (SPE), and immunoaffinity chromatography.

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  • Determination using reversed-phase liquid chromatography (LC) with fluorescence or tandem mass spectrometry detection, and ELISA methods.

    • Main Results:

    • Immunoaffinity chromatography enables detection of sub-ppb levels of OTA in diverse food matrices and plasma.
    • Reversed-phase LC with fluorescence detection is a widely adopted method.
    • Tandem mass spectrometry offers a more recent and sensitive detection approach.

    Conclusions:

    • Reliable and sensitive methods are essential for regulating ochratoxin A in food.
    • Immunoaffinity chromatography coupled with LC-fluorescence or LC-MS/MS provides effective tools for OTA analysis.
    • Availability of certified reference materials supports accurate OTA quantification.