Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A high-throughput continuous sample introduction interface for microfluidic chip-based capillary electrophoresis

Qun Fang1, Guang-Ming Xu, Zhao-Lun Fang

  • 1Institute of Microanalytical Systems, Chemistry Department, Zhejiang University, Hangzhou, China.

Analytical Chemistry
|April 2, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Personalizing post-stroke symptom management: integrating network analysis and in silico intervention to identify subgroup-specific targets.

BMC neurology·2026
Same author

Exercise modulates food reward: neurobiological mechanisms and implications for weight management.

Frontiers in nutrition·2026
Same author

Letter to the Editor in response to the article "Association between thyroid dysfunction and cognitive impairment in type 2 diabetes mellitus: A cross-sectional study".

Journal of diabetes investigation·2026
Same author

Single-Cell Proteomics Decodes the Cellular Response to Lysosomal Storage in <i>C. elegans</i> Coelomocytes.

International journal of molecular sciences·2026
Same author

Simultaneous In-Depth Single-Cell Proteomic and Metabolomic Analysis.

Analytical chemistry·2026
Same author

The Reorganization of Cerebellar Functional Network Topology in Post-stroke Aphasia: A Resting-State fMRI Study.

Molecular neurobiology·2026
Same journal

Machine Learning-Assisted Label-Free SERS Decoding of Mitochondrial Molecular Dynamics in Ovarian Granulosa Cells during Aging.

Analytical chemistry·2026
Same journal

Revealing the Regulatory Interplay of NHE1 mRNA and Na<sup>+</sup> in Cancer Cells Using a DNA Nanosensor.

Analytical chemistry·2026
Same journal

Towards Cellular Resolution of Tryptic Peptides in Tissue Sections by MALDI MS Imaging: A Focus on Enzyme Application and Reproducibility.

Analytical chemistry·2026
Same journal

Bioinspired Bilayer Hydrogel Colorimetric Sensor Array for Low-Temperature Food Freshness Analysis.

Analytical chemistry·2026
Same journal

Quartz Crystal Microbalance-Based Point-of-Care Testing Systems: Principles, Device Design, and Applications.

Analytical chemistry·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
See all related articles

This study introduces a novel flow-through sampling reservoir for microfluidic chips, enabling efficient sample introduction for capillary electrophoresis. The system achieved high throughput and precision in amino acid analysis, demonstrating its utility for real-life sample applications.

Area of Science:

  • Analytical Chemistry
  • Microfluidics
  • Biotechnology

Background:

  • Efficient sample introduction is crucial for microfluidic analytical systems to analyze real-world samples.
  • Current methods often face challenges with sample handling and carryover in microfluidic devices.

Purpose of the Study:

  • To develop and evaluate a novel flow-through sampling reservoir for seamless world-to-chip interfacing in microfluidic capillary electrophoresis.
  • To demonstrate the system's capability for high-throughput and precise analysis of multiple samples without interruption.

Main Methods:

  • Fabrication of a flow-through reservoir on a planar glass chip with a guided overflow design.
  • Utilizing air-segmented sample injection for efficient sample change at a flow rate of 0.48 mL/min.

Related Experiment Videos

  • Capillary electrophoresis separation with Laser-Induced Fluorescence (LIF) detection for FITC-labeled amino acids.

    • Main Results:

    • Achieved a maximum throughput of 80 samples per hour with less than 4.1% carryover.
    • Demonstrated high precision for amino acid separation, with relative standard deviations (RSD) ranging from 1.5% to 2.6%.
    • Successfully monitored amino acid derivatization over 4 hours with an overall precision of 4.8% RSD for the internal standard.

    Conclusions:

    • The novel flow-through sampling reservoir effectively addresses world-to-chip interfacing challenges in microfluidics.
    • The system offers high throughput, precision, and robustness for continuous analysis of real-life samples.
    • This technology holds significant potential for various applications in analytical chemistry and biotechnology.