Metabolism of estrogens and thier sulfates by mouse kidney tissue in vitro

Whole kidney tissue from mouse was incubated with labelled estrone 3-sulfate (E13S), 17beta-estradiol 3-sulfate (17betaE23S), estrone (E1), and 17beta-estradiol (17betaE2). Considerable reduction of E13S and E1 occurred. E13S gave rise primarily to 17alpha-estradiol 3-sulfate (17 alphaE23S) together with lesser amounts of 17betaE23S. By incubating [3H-35S]E13S with the tissue it was confirmed that formation of the diol sulfates was direct, without accompanying hydrolysis and reconjugation. Conversion of E1 was mainly to 17betaE2 with, on the average, lesser amounts of 17alphaE2. A small degree of direct conversion of 17betaE23S to E13S was found. 17beta-Estradiol was converted to a limited extent to E1 and to much smaller amounts of 17alphaE2.