Related Experiment Video
Updated: Sep 30, 2026
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
Epitope analysis of human immunoglobulin D with a mouse monoclonal anti-Fab delta chain antibody
Masaaki Arakawa1, Yoshihisa Itoh, Naofumi Yoshioka
1Biochemical Research Laboratory, Research and Development Headquarters, Eiken Chemical Co., Ltd., 143 Nogi, Nogi-machi, Shimotsuga-gun, Tochigi-ken, 329-0114, Japan. massaaki_arakawa@eiken.co.jp
Background:
The clinical significance of IgD measurements in serum is limited as the frequency of abnormal concentrations is rare.
Methods:
We prepared a mouse monoclonal antibody and a rabbit polyclonal antibody against Fab delta and Fc delta chain and compared epitope recognition by the monoclonal antibody against Fab delta (anti-Fab delta mono) with that by other antibodies.
Results:
Anti-Fab delta mono specifically reacted with purified IgD and Fab delta of myelomatous origin, but not with other isotypes and light chains. In 19 of 22 myeloma sera, the monoclonal antibody recognized intact IgD and/or its fragments when analyzed by immunoblotting. Of these there were only four cases in which possible Fab delta fragments were identified. The other three sera showed no reactivity with the antibody and the IgD value was low on a chemiluminescence enzyme immunoassay.
Conclusions:
These findings indicate the presence of at least two immunochemically different IgD molecules in the sera. No positive reaction with any synthetic peptide was observed for the antibody on delta-chain ranging from N-terminus of JH, C delta 1 to a hinge region. We suggest that the epitope recognized by the antibody is related to the variable region or a conformational structure on the Fd delta region of IgD.
Related Concept Videos
01:10Antibody Structure
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
01:10Antibody Structure
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...