Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Dax1 expression is dependent on steroidogenic factor 1 in the developing gonad.

Christine Hoyle1, Veronica Narvaez, Graham Alldus

  • 1Section of Gene Function and Regulation, Chester Beatty Laboratories, Institute of Cancer Research, London SW3 6JB.

Molecular Endocrinology (Baltimore, Md.)
|March 30, 2002
PubMed
Summary

Researchers identified a key DNA region regulating Dax1 gene expression in embryonic gonads. This finding reveals how steroidogenic factor 1 (SF1) controls Dax1, crucial for mammalian gonad development and sex determination.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

BDNF regulates pituitary stem cell engagement toward precursor state.

Stem cell reports·2026
Same author

Srr2-dependent SOX2 levels govern the chromatin and transcriptional landscape of adult neural stem cell fate decisions in mouse.

Genome biology·2026
Same author

NR3C1 is required for normal somatotrope differentiation and Foxo1 expression in pituitary.

Endocrinology·2026
Same author

BDNF Regulates Pituitary Stem Cell Engagement towards precursor state.

bioRxiv : the preprint server for biology·2026
Same author

Modeling the chondrocyte-derived osteoblasts formation process reveals its molecular signature and regulation network.

Bone research·2026
Same author

Publisher Correction: Diversity and immune dynamics of choroid plexus macrophages are shaped by distinct developmental origins.

Nature neuroscience·2026

Area of Science:

  • Developmental Biology
  • Genetics
  • Endocrinology

Background:

  • The nuclear hormone receptor DAX1 plays a vital role in mammalian gonad development and sex determination.
  • DAX1 gene expression exhibits dynamic temporal and spatial patterns within embryonic and adult gonads.

Purpose of the Study:

  • To investigate the in vivo regulation of Dax1 gene expression during embryonic gonad development.
  • To identify specific regulatory elements controlling Dax1 expression in the developing gonad.

Main Methods:

  • Utilized a transgenic mouse model to study Dax1 gene regulation.
  • Employed a LacZ reporter gene assay to identify essential regulatory regions.
  • Analyzed Dax1 expression levels in SF1-deficient embryos.

Related Experiment Videos

Main Results:

  • Identified a novel 500-bp upstream region critical for embryonic gonad LacZ expression.
  • A conserved steroidogenic factor 1 (SF1) binding site within this region directs expression to the embryonic gonad.
  • Dax1 expression was significantly reduced in SF1-deficient embryonic gonads.
  • SF1 binding sites near the Dax1 start codon are important for regulating expression levels.

Conclusions:

  • Identified a critical in vivo regulatory region for early gonad Dax1 expression.
  • Demonstrated the essential role of SF1 in regulating Dax1 during gonad development.
  • Provided novel insights into enhancer element function across different cell types and developmental stages.