Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Profiling alternative splicing on fiber-optic arrays.

Joanne M Yeakley1, Jian-Bing Fan, Dennis Doucet

  • 1Department of Cellular and Molecular Medicine, University of California-San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.

Nature Biotechnology
|March 30, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of clinical-radiological and radiomics features for predicting pulmonary nodule malignancy in a multicenter study of mixed clinical and surveillance populations.

European radiology·2026
Same author

An Approach for Differential Diagnosis of Breast Tumors by ctDNA Methylation Sequencing.

Cancer medicine·2025
Same author

A urine DNA methylation assay for early detection of renal cancer.

Oncogene·2025
Same author

Enhanced HER2 status detection in breast and gastric cancers using surrogate DNA methylation markers.

IUBMB life·2025
Same author

High-throughput gene expression analysis with TempO-LINC sensitively resolves complex brain, lung and kidney heterogeneity at single-cell resolution.

Scientific reports·2024
Same author

General anesthesia induces acute cell-free DNA methylation changes in peripheral blood.

Journal of thoracic disease·2024
Same journal

Author Correction: Improved RNA base editing with guide RNAs mimicking highly edited endogenous ADAR substrates.

Nature biotechnology·2026
Same journal

Unlocking the chemical potential of filamentous fungi using prime editing.

Nature biotechnology·2026
Same journal

A genome-scale CRISPRi perturbation atlas of human induced pluripotent stem cells.

Nature biotechnology·2026
Same journal

Prime editing for precise genome engineering and modulation of fungal metabolism.

Nature biotechnology·2026
Same journal

Retargeted serine integrases for one-step, precise integration of large DNA sequences in human cells.

Nature biotechnology·2026
Same journal

A retargeted recombinase for precise insertion of large DNA.

Nature biotechnology·2026
See all related articles

This study introduces a novel assay for analyzing messenger RNA (mRNA) isoforms, simplifying the detection of alternative splicing. The method offers sensitive and specific parallel analysis directly from cellular RNA, aiding disease research.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • The human transcriptome exhibits complex alternative mRNA splicing and numerous related genes.
  • Distinguishing these closely related transcripts is challenging with standard microarray methods.

Purpose of the Study:

  • To develop a sensitive and specific assay for parallel analysis of mRNA isoforms.
  • To enable mRNA transcript analysis without prior RNA purification or cDNA synthesis.

Main Methods:

  • A fiber-optic microarray platform was utilized for parallel mRNA isoform analysis.
  • The assay was validated using an endogenously expressed viral transcript model.
  • Analysis was performed directly on total cellular RNA or from a few cells.

Related Experiment Videos

Main Results:

  • The assay detected mRNA isoforms from as little as 10-100 pg of total cellular RNA.
  • Multiplexed analysis of human cancer cell lines revealed differences in mRNA splicing.
  • A potential autocrine mechanism in choriocarcinoma development was suggested.

Conclusions:

  • This approach provides a sensitive and specific method for parallel mRNA isoform analysis.
  • The assay facilitates large-scale studies on alternative splicing in development and disease.
  • It offers a valuable tool for cancer research, particularly in understanding choriocarcinoma development.