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Sequencing and chromosomal assignment of the rat endothelial-derived lipase gene (Lipg)
A C Bonné1, M G Den Bieman, H A Van Lith
1Department of Laboratory Animal Science, Veterinary Faculty, Utrecht University, The Netherlands. bonne@las.vet.uu.nl
DNA Sequence : the Journal of DNA Sequencing and Mapping
|April 2, 2002
Abstract:
Part of the nucleotide sequence of the Lipg gene in the rat was established using primers based on the mRNA sequence described in the mouse. The rat intron sequence served as a template for designing primers for the specific amplification of rat Lipg. A rat-hamster radiation hybrid (RH) panel was used for chromosomal assignment of the rat Lipg gene. The Lipg gene was found to be located on rat chromosome 18 in the vicinity of the marker D18Mit11; a region reported to be homologous with both human and mouse chromosome 18.