Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genetic engineering for xenotransplantation.

M S Sandrin1, B E Loveland, I F McKenzie

  • 1Molecular Immunogenetics Laboratory, The Austin Research Institute, Heidelberg, Victoria, Australia. m.sandrin@ari.unimelb.edu.au

Journal of Cardiac Surgery
|April 2, 2002
PubMed
Summary

Xenotransplantation research addresses organ shortages by overcoming immune barriers like the Gal antigen and coagulation issues. Genetic modifications in pigs are key to advancing xenograft survival and therapeutic potential.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Testicular immune cell populations and macrophage polarisation in adult male mice and the influence of altered activin A levels.

Journal of reproductive immunology·2020
Same author

An in vitro model demonstrates the potential of neoplastic human germ cells to influence the tumour microenvironment.

Andrology·2017
Same author

Long-term controlled normoglycemia in diabetic non-human primates after transplantation with hCD46 transgenic porcine islets.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons·2009
Same author

Human dendritic cells pulsed with specific lipopeptides stimulate autologous antigen-specific T cells without the addition of exogenous maturation factors.

Journal of viral hepatitis·2008
Same author

A therapeutic human anti-idiotypic antibody mimics CD55 in three distinct regions.

European journal of immunology·2000
Same author

Are pigs transgenic for human complement regulatory proteins necessary for xenotransplantation?

Transplantation·2000

Area of Science:

  • Transplantation immunology
  • Genetics
  • Biomedical engineering

Background:

  • Organ transplantation faces a critical donor shortage, driving research into xenotransplantation.
  • Hyperacute rejection was a major hurdle, but experimental studies on the Gal antigen and complement regulation have enabled initial xenograft survival.
  • Beyond immune responses, molecular incompatibilities affecting cell surface interactions also contribute to xenograft failure.

Purpose of the Study:

  • To explore strategies for overcoming barriers in xenotransplantation.
  • To investigate the role of genetic modification in enhancing xenograft survival.
  • To identify and address molecular incompatibilities beyond immune rejection.

Main Methods:

  • Experimental xenotransplantation models.

Related Experiment Videos

  • Genetic engineering, including gene knockout and transgenic approaches (e.g., expressing human complement-regulating proteins).
  • Enzymatic modification of cell surface carbohydrates to reduce xenoantigens.

    • Main Results:

    • Eliminating or reducing the Gal antigen and controlling complement activation improve xenograft survival.
    • Unregulated coagulation and cellular reactions (macrophages, neutrophils, NK cells, T lymphocytes) remain significant barriers.
    • Transgenic strategies, particularly in pigs, show promise for controlling antigen expression, complement, and coagulation.
    • Expression of human complement-regulating proteins in pigs highlights the need to address coagulation system dysregulation.

    Conclusions:

    • Xenotransplantation offers a potential solution to the organ donor shortage.
    • Overcoming xenograft rejection requires addressing both immunological and non-immunological barriers, including coagulation and molecular incompatibilities.
    • Advanced genetic engineering techniques, especially in pigs, are crucial for developing effective xenotransplantation strategies.