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Oxybuprocaine induces a false-positive response in immunochromatographic SAS Adeno Test
Takeshi Hoshino1, Taiji Takanashi, Morio Okada
1Department of Ophthalmology, Kurashiki Central Hospital, Kurashiki, Japan.
Objective:
To investigate whether a solution of oxybuprocaine hydrochloride, 0.4%, results in a false-positive response in an immunochromatographic SAS Adeno Test.
Design:
Experimental study.
Controls:
Physiologic saline and 2% lidocaine.
Testing:
Each chemical (100 microl) was diluted in a transport medium. Five drops (200 microl) of the resultant solution were dispensed into the round sample well of a test device. Fifteen samples were tested in each group.
Main Outcome Measures:
Ten minutes after the start of the test, a colored line in the "specimen" portion of the test membrane was visually read as positive or negative by a masked technician.
Results:
No positive reaction was observed in the control groups (physiologic saline and lidocaine). A false-positive reaction was observed in six samples (33.3%) in the oxybuprocaine group. The positive rate was significantly higher in the oxybuprocaine group compared with those in control groups (P = 0.0062, Fisher's extract probability test).
Conclusions:
Oxybuprocaine may induce a false-positive reaction in an immunochromatographic SAS Adeno Test. We recommend the use of lidocaine, instead of oxybuprocaine, for local anesthesia in taking eye swabs from patients with suspected adenovirus infection.