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Updated: Oct 1, 2026

An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants
Published on: June 12, 2026
[Validation of the HPLC method in the determination of dioxopromethazine and phenylephrine in eye drops]
T Hudecová1, S Hatrík, N Zimová
1Katedra farmaceutickej analýzy a nukleárnej farmácie Farmaceutickej fakulty Univerzity Komenského, Bratislava. hudecova@fpharm.uniba.sk
Abstract:
The present paper introduces a rapid HPLC method for the determination of dioxopromethazine and phenylephrine in eye drops. The method uses a modified C18 stationary phase optimized for the separation of basic compounds and a methanol/1.5 mM phosphoric acid (60/40 v/v, pH 3.02) mobile phase. The flow rate is set to 2 ml/min, sample volume 20 microliters, and compounds are detected at 275 nm. Prior to analysis, the eye drops are diluted with water in a ratio of 1:50. The elaborated HPLC method and the chromatographic system were validated according to the procedure for the validation of chromatographic systems and methods.
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