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Related Experiment Videos

Sialylation is essential for early development in mice.

Martina Schwarzkopf1, Klaus-Peter Knobeloch, Elvira Rohde

  • 1Institut für Molekularbiologie und Biochemie, Fachbereich Humanmedizin, Freie Universität Berlin, Arnimallee 22, D-14195 Berlin-Dahlem, Germany.

Proceedings of the National Academy of Sciences of the United States of America
|April 4, 2002
PubMed
Summary

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Inactivation of the UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (UDP-GlcNAc 2-epimerase) enzyme causes early embryonic lethality in mice. This highlights the critical role of sialylation in embryonic development.

Area of Science:

  • Biochemistry
  • Developmental Biology
  • Glycobiology

Background:

  • Sialic acids are terminal carbohydrates on eukaryotic cell glycoconjugates, essential for cellular functions like adhesion and signal recognition.
  • Sialylation regulates glycoprotein stability and is vital for various biological processes.
  • The bifunctional UDP-N-acetylglucosamine-2-epimerase/N-acetylmannosamine kinase (UDP-GlcNAc 2-epimerase) is the key enzyme in sialic acid biosynthesis.

Purpose of the Study:

  • To investigate the essential role of UDP-GlcNAc 2-epimerase in sialic acid biosynthesis and its impact on embryonic development.
  • To demonstrate the fundamental importance of sialylation during early development.

Main Methods:

  • Gene targeting was employed to inactivate the UDP-GlcNAc 2-epimerase gene in mice.

Related Experiment Videos

  • Embryonic stem cells were utilized to study the polysialylation of the neural cell adhesion molecule.

    • Main Results:

    • Inactivation of UDP-GlcNAc 2-epimerase resulted in early embryonic lethality in mice.
    • The study demonstrated the necessity of UDP-GlcNAc 2-epimerase for proper sialylation processes, exemplified by neural cell adhesion molecule polysialylation.

    Conclusions:

    • The bifunctional UDP-GlcNAc 2-epimerase is indispensable for embryonic development due to its role in sialic acid biosynthesis.
    • Sialylation, regulated by UDP-GlcNAc 2-epimerase, is fundamentally required for successful embryonic development.