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Updated: Oct 1, 2026

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
Published on: February 13, 2012
[Effect of SP on intracellular free calcium concentration in cultured osteoblast]
1Department of Orthodontics, College of Stomatology, Fourth Military Medical University, Xi'an 710032, China.
Objective:
To investigate the effect of SP on intracellular free calcium concentration (Ca2+)i in cultured osteoblast.
Methods:
Effect of SP on intracellular free calcium was measured using cell culturing and laser scanning confocal microscopy with fluo-3/AM as a fluorescence probe.
Results:
After treated with SP(10(-4) mol/L), 70% of the cells responded to it; the intracellular (Ca2+)i increased markedly [fluorescence intensity increased by (90 +/- 18)%].
Conclusions:
These results suggested that calcium may have a role as a intracellular secondary messenger in the action of SP in osteoblast.
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