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Reparable sonoporation generated by microstreaming.

Junru Wu1, Joel P Ross, Jen-Fu Chiu

  • 1Department of Physics, University of Vermont, Burlington 05405, USA. jwu@zoo.uvm.edu

The Journal of the Acoustical Society of America
|April 5, 2002
PubMed
Summary
This summary is machine-generated.

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This study shows that shear stress from microstreaming around a vibrating horn causes reparable sonoporation in Jurkat lymphocytes. Shorter exposure times require higher shear stress thresholds for this cell membrane poration.

Area of Science:

  • Biophysics
  • Cell Biology
  • Acoustic Science

Background:

  • Sonoporation is a method for increasing cell membrane permeability.
  • Understanding the biophysical mechanisms of sonoporation is crucial for its applications.

Purpose of the Study:

  • To investigate the mechanism of reparable sonoporation in Jurkat lymphocytes induced by a vibrating horn.
  • To determine the threshold shear stress for sonoporation and its relationship with exposure time.

Main Methods:

  • Jurkat lymphocytes in suspension were exposed to a vibrating Mason horn (21.4 KHz, 400 microm tip diameter, 7.8 microm amplitude).
  • The role of shear stress and microstreaming in cell membrane poration was analyzed.
  • Threshold shear stress for reparable sonoporation was quantified at different exposure times.

Related Experiment Videos

Main Results:

  • Reparable sonoporation was successfully induced in Jurkat lymphocytes.
  • Shear stress generated by microstreaming around the horn tip was identified as the primary mechanism.
  • The threshold shear stress was determined to be 12 +/- 4 Pa for exposure up to 7 minutes.
  • A shorter exposure time correlated with a greater threshold shear stress.

Conclusions:

  • Microstreaming-induced shear stress is the key factor in reparable sonoporation of lymphocytes.
  • The findings provide quantitative data on shear stress thresholds for sonoporation.
  • This research contributes to understanding acoustic effects on cell membranes for potential biotechnological applications.