Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Extrapituitary TSH in early chick embryos: Pit-1 dependence?

Amy E Murphy1, Steve Harvey

  • 1Department of Physiology, Perinatal Research Centre, University of Alberta, Edmonton, Canada.

Journal of Molecular Neuroscience : MN
|April 5, 2002
PubMed
Summary

Thyrotropin (TSH) gene expression in chick embryos shows widespread TSH presence in extrapituitary tissues. Pituitary-specific transcription factor Pit-1 involvement in TSH expression is tissue-specific and not universal.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An extremely rare case of multiple small bowel intussusceptions in a 20-year-old female.

Journal of surgical case reports·2024
Same author

Acute Pancreatitis: Exploring Nutrition Implications.

Nutrition in clinical practice : official publication of the American Society for Parenteral and Enteral Nutrition·2020
Same author

Growth Hormone Neuroprotection Against Kainate Excitotoxicity in the Retina is Mediated by Notch/PTEN/Akt Signaling.

Investigative ophthalmology & visual science·2019
Same author

Regenerative Effect of Growth Hormone (GH) in the Retina after Kainic Acid Excitotoxic Damage.

International journal of molecular sciences·2019
Same author

Trends and Outcomes of Synchronous Resection of Colorectal Metastasis in the Modern Era-Analysis of Targeted Hepatic NSQIP Database.

The Journal of surgical research·2019
Same author

Thirty-Day Readmission Rate Using an Outpatient Infusion Pathway after Laparoscopic Roux-en-Y Gastric Bypass.

The American surgeon·2018

Area of Science:

  • Developmental Biology
  • Endocrinology
  • Molecular Biology

Background:

  • Pituitary-specific transcription factor Pit-1 is considered crucial for thyrotropin (TSH) gene expression in the pituitary gland.
  • TSH immunoreactivity has been observed in various extrapituitary tissues, suggesting potential roles beyond the pituitary.
  • The presence and function of Pit-1 in extrapituitary tissues during embryonic development remain largely unexplored.

Purpose of the Study:

  • To investigate the distribution of Pit-1 and TSH immunoreactivity in embryonic chick tissues.
  • To determine the potential involvement of Pit-1 in the extrapituitary expression of TSH prior to pituitary differentiation.
  • To assess whether Pit-1's role in TSH expression is conserved across different embryonic tissues.

Main Methods:

Related Experiment Videos

  • Immunohistochemical analysis was performed on embryonic chick tissues at the end of the first trimester (21-day incubation).
  • Antibodies against TSH and Pit-1 were used to detect their respective immunoreactivities in various tissues, including brain, spinal cord, liver, lungs, gut, and limb buds.
  • Co-localization studies were conducted to compare the distribution patterns of TSH and Pit-1.

Main Results:

  • TSH immunoreactivity was detected in discrete cells across multiple extrapituitary tissues, including brain, spinal cord, liver, lungs, gut, and limb buds.
  • Co-localization of TSH and Pit-1 immunoreactivity was observed in specific tissues like brain and spinal cord ependymal cells and liver hepatocytes, suggesting Pit-1-dependent TSH expression.
  • However, several tissues exhibited intense TSH staining with minimal Pit-1 immunoreactivity (e.g., skin, limb bud, bronchus), indicating Pit-1-independent TSH gene expression.

Conclusions:

  • Pit-1 is present in both pituitary and extrapituitary tissues of embryonic chickens.
  • The involvement of Pit-1 in extrapituitary TSH expression in chick embryos is tissue-specific.
  • These findings highlight a complex regulatory mechanism for TSH gene expression during embryonic development, with both Pit-1-dependent and Pit-1-independent pathways.