Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ty1 defect in proteolysis at high temperature.

Joseph F Lawler1, Daniel P Haeusser, Angie Dull

  • 1Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.

Journal of Virology
|April 5, 2002
PubMed
Summary

High temperatures abolish Ty1 retrotransposition in Saccharomyces cerevisiae by inhibiting Gag-Pol-p199 polyprotein processing and reducing reverse transcriptase (RT) activity. These protein alterations disrupt both protease and RT functions, halting retrotransposition.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The oxidative cost of losing low temperature viability protein 1 [Ltv1]: insights into cellular damage and homeostasis.

FEMS yeast research·2026
Same author

Genome-wide histone humanization in yeast disrupts genome organization, replication, and rDNA stability.

Cell reports·2026
Same author

Author Correction: Physiology and immunology of a pig-to-human decedent kidney xenotransplant.

Nature·2026
Same author

Improved vector toolkit for genome writing in mammalian cells.

bioRxiv : the preprint server for biology·2026
Same author

Retrotransposon Activation in the Aged and Alzheimer's Disease Brain Examined by Nanopore Long-read DNA Sequencing.

bioRxiv : the preprint server for biology·2026
Same author

To make biology programmable, we must master its generative grammar.

Molecular therapy : the journal of the American Society of Gene Therapy·2026

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Retrotransposition Mechanisms

Background:

  • The Ty1 element in Saccharomyces cerevisiae is a retrotransposant.
  • Ty1 retrotransposition exhibits temperature sensitivity, ceasing above 34°C.

Purpose of the Study:

  • To elucidate the molecular mechanisms underlying temperature-sensitive inhibition of Ty1 retrotransposition.
  • To investigate the impact of high temperatures on Ty1 protein processing and enzymatic activities.

Main Methods:

  • Expression of Ty1 protease construct in Escherichia coli.
  • Analysis of Gag and Gag-Pol polyprotein processing in yeast at high temperatures.
  • Assessment of reverse transcriptase (RT) activity in virus-like particles.

Related Experiment Videos

Main Results:

  • High temperature inhibits Gag-Pol-p199 polyprotein processing and reduces reverse transcriptase (RT) activity.
  • Ty1 protease exhibits inherent temperature sensitivity.
  • Gag processing is partially inhibited, while Gag-Pol cleavage is completely inhibited at high temperatures.
  • RT activity is significantly reduced in virus-like particles formed at high temperatures.

Conclusions:

  • Temperature-sensitive alterations in Ty1 proteins affect both protease and RT activities.
  • These combined effects lead to the abolition of Ty1 transposition at elevated temperatures.