Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Somatic cell genetic analysis of the interferon system.

R P Creagan, Y H Tan, S Chen

    Federation Proceedings
    |December 1, 1975
    PubMed
    Summary

    Somatic cell hybrids help study human interferon production and regulation. Mouse-human hybrid cells mapped interferon genes to chromosomes 2, 5, 16, and 21.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Repression of TFII-I-dependent transcription by nuclear exclusion.

    Proceedings of the National Academy of Sciences of the United States of America·2001
    Same author

    An efficient cis-element discovery method using multiple sequence comparisons based on evolutionary relationships.

    Genomics·2001
    Same author

    Isolation and characterization of BEN, a member of the TFII-I family of DNA-binding proteins containing distinct helix-loop-helix domains.

    Proceedings of the National Academy of Sciences of the United States of America·2000
    Same author

    A recombinogenic targeting method to modify large-inserts for cis-regulatory analysis in transgenic mice: construction and expression of a 100-kb, zebrafish Hoxa-11b-lacZ reporter gene.

    Development genes and evolution·2000
    Same author

    Direct cloning of genomic DNA by recombinogenic targeting method using a yeast-bacterial shuttle vector, pClasper.

    Genomics·1999
    Same author

    Mammalian Trithorax and polycomb-group homologues are antagonistic regulators of homeotic development.

    Proceedings of the National Academy of Sciences of the United States of America·1999

    Area of Science:

    • Immunology
    • Genetics
    • Cell Biology

    Background:

    • Somatic cell hybrid systems are valuable tools for studying eukaryotic cell functions.
    • Investigating the human interferon system using somatic cells has advanced understanding of cellular defense mechanisms.

    Purpose of the Study:

    • To review the application of somatic cell hybrid systems in studying the human interferon system.
    • To assign human genes involved in interferon production and regulation to specific chromosomes.

    Main Methods:

    • Utilized interspecific heterokaryons and hybrid cells for interferon production studies.
    • Employed mouse-human hybrid cells to map human interferon-related genes.

    Main Results:

    • Hybrid cells can produce interferons of both parental types and offer protection against viral challenges.
    • Human genes regulating interferon were assigned to chromosome 21.
    • Genes for human interferon production were mapped to chromosomes 2 and 5.
    • A locus controlling interferon production may be on chromosome 16.

    Conclusions:

    • Somatic cell hybridization is effective for human gene mapping within the interferon system.
    • Further applications include studying interferon structure and developing high-yield interferon-producing hybrid lines.

    Related Experiment Videos