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Modeling pore size distribution in cellulose rolled stationary phases
Craig Keim1, Chenghong Li, Christine M Ladisch
1Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
Biotechnology Progress
|April 6, 2002
Summary
Rolled stationary phases, a type of fabric, can separate proteins using size exclusion chromatography. A logistic model effectively describes their pore size distribution, which changes predictably after enzyme treatment.
Area of Science:
- Chromatography
- Materials Science
- Biochemistry
Background:
- Rolled stationary phases are nonparticulate materials used for rapid protein separation.
- Their fabric structure presents an atypical matrix for size exclusion chromatography.
- Understanding pore size distribution is crucial for chromatographic performance.
Purpose of the Study:
- To investigate the potential of rolled stationary phases for size exclusion chromatography.
- To model the pore size distribution of these fabric-based stationary phases.
- To analyze the impact of enzyme treatment on pore characteristics and chromatographic behavior.
Main Methods:
- Utilized various probes (PEG, Dextran, D2O, glucose, NaCl) to measure pore sizes.
- Applied a logistic model to characterize pore size distributions.
- Performed enzyme (cellulase) treatment on the fabric to alter pore structure.
Main Results:
- A simple logistic model accurately described the pore size distribution of the rolled stationary phase.
- Enzyme treatment led to a uniform decrease in water-accessible pores.
- Peak retention decreased following enzyme treatment, consistent with pore size changes.
Conclusions:
- Rolled stationary phases demonstrate viable size exclusion chromatography capabilities.
- The logistic function is a suitable model for characterizing and comparing pore size distributions.
- Enzyme modification offers a method to tune the chromatographic properties of fabric-based stationary phases.
