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Anisylazoformylarginine: a superior assay substrate for carboxypeptidase B type enzymes
William L Mock1, Daniel J Stanford
1Department of Chemistry, University of Illinois at Chicago, Chicago, IL 60607-7061, USA. wlmock@uic.edu
Bioorganic & Medicinal Chemistry Letters
|April 6, 2002
Abstract:
Anisylazoformylarginine (CH(3)OC(6)H(4)-N=N-CO-Arg-OH) is rapidly hydrolyzed at the acyl-arginine linkage by the zinc-enzyme porcine carboxypeptidase B. The catalytic reaction is readily monitored spectrophotometrically by disappearance of the intense absorption (349 nm, epsilon 19,100) of the azo chromophore, which chemically fragments after substrate cleavage.