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Published on: February 8, 2019
Biodiversity (Communications arising): maize transgene results in Mexico are artefacts
Nick Kaplinsky1, David Braun, Damon Lisch
1Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA. nkaplins@nature.berkeley.edu
Abstract:
Quist and Chapela's conclusion that the transgenes they claim to have detected in native maize in Oaxaca, Mexico, are predominantly reassorted and inserted into a "diversity of genomic contexts" seems to be based on an artefact arising from the inverse polymerase chain reaction (i-PCR) they used to amplify sequences flanking 35S transgenes from cauliflower mosaic virus (CaMV).
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