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Golgi apparatus and TGN during endocytosis
Monika Vetterlein1, Adolf Ellinger, Josef Neumüller
1Institute of Histology and Embryology, Department of Cell Biology and Ultrastructure Research, University of Vienna, Schwarzspanierstrasse 17, 1090 Vienna, Austria.
Histochemistry and Cell Biology
|April 6, 2002
Summary
Wheat germ agglutinin (WGA) uptake involves dynamic membrane remodeling at the trans-Golgi network (TGN). This process newly forms, expands, and integrates endocytic TGNs into Golgi stacks for WGA internalization.
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Endocytosis is a fundamental cellular process for internalizing extracellular material.
- The Golgi apparatus plays a crucial role in protein modification and sorting.
- Understanding the dynamics of endosomal trafficking to the Golgi is essential for cell function.
Purpose of the Study:
- To analyze the endosomal compartments and Golgi apparatus during wheat germ agglutinin (WGA) endocytosis.
- To elucidate the stages and membrane dynamics involved in WGA transport to the Golgi.
Main Methods:
- Utilized wheat germ agglutinin labeled with horseradish peroxidase (WGA-HRP) in HepG(2) hepatoma cells.
- Observed early and late periods of endocytosis using microscopy to track WGA localization.
Main Results:
- WGA rapidly translocated to the Golgi region, undergoing a three-stage transport process.
- Stage II involved the formation of extended endocytic trans-Golgi networks (TGNs) associated with the endoplasmic reticulum.
- Endocytic TGNs were integrated into Golgi stacks, with stacked cisternae becoming prominent endocytic compartments in Stage III.
Conclusions:
- Endocytosis of WGA involves significant membrane dynamics at the trans-Golgi side, including de novo TGN formation and subsequent consumption.
- Incorporation of TGN elements into Golgi stacks appears to be a key mechanism for internalizing WGA into the Golgi apparatus.