Enzyme sensor array for the determination of biogenic amines in food samples

Area of Science:

• Analytical Chemistry
• Biosensor Technology
• Food Science

Background:

• Biogenic amines are critical indicators of food quality and safety.
• Accurate and rapid detection methods for multiple biogenic amines are needed in the food industry.

Purpose of the Study:

• To develop and validate an enzyme sensor array for simultaneous determination of histamine, tyramine, and putrescine.
• To apply the sensor array to diverse food matrices and compare results with conventional methods.

Main Methods:

• Immobilization of monoamine oxidase, tyramine oxidase, and diamine oxidase on screen-printed electrodes.
• Utilizing an artificial neural network for pattern recognition and quantification of biogenic amines.
• Testing the sensor array on various food samples including fish, meat, dairy, and fermented products.

Main Results:

• The enzyme sensor array achieved detection limits of 10 mg/kg (histamine, tyramine) and 5 mg/kg (putrescine).
• Linear ranges were up to 200 mg/kg (histamine, tyramine) and 100 mg/kg (putrescine).
• High correlation (0.854) was observed when compared to conventional Liquid Chromatography (LC) analyses across multiple food types.

Conclusions:

• The developed enzyme sensor array offers a rapid (20 min) and reliable method for simultaneous biogenic amine analysis in food.
• This biosensor provides a viable alternative to traditional analytical techniques for food quality control.