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Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
A simplified method for the HPLC resolution of alpha-carotene and beta-carotene (trans and cis) isomers
M Bononi1, I Commissati, E Lubian
1Laboratorio Analisi Alimenti, Facoltà di Agraria, Università degli Studi di Milano, Italy.
Abstract:
A new HPLC/DAD (Diode Array Detector) method is proposed for the identification of some carotene isomers. The operating conditions adopted permit the resolution of alpha-carotene, all-trans-beta-carotene, 9-cis-beta-carotene, 13-cis-beta-carotene and 15-cis-beta-carotene. Moreover, the chromatographic conditions reported are simplified in respect of those reported up to now. The method is applied to the determination of carotenoids in a dried Dunaliella salina extract, but it could be also applied to other organic matrices such as eggs.
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