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Mapping of epitopes on the fiber knobs of human adenovirus serotypes 8 and 15
Herbert Liebermann1, Kristina Lotz, Werner Seidel
1Friedrich-Loeffler-Institut für Medizinische Mikrobiologie, Ernst-Moritz-Arndt-Universität Greifswald, Greifswald, Deutschland.
Abstract:
In order to obtain information on the linear antigenic epitopes on fiber knobs of adenovirus serotypes 8 (Ad8) and 15 (Ad15) of subgenus D, the binding of polyclonal virus- and fiber-specific rabbit antibodies to overlapping peptides covering the fiber knob was studied. The main antigenic epitopes of the fiber knob of Ad8 (FK8) were represented by the peptides P4 [amino acids (aa) 213-227], P6 (aa 233-247), P11 (aa 283-297), P13 (aa 303-317) and P15 (aa 316-325); the peptides P1 (aa 183-197), P8 (aa 253-267), P10 (aa 273-287) and P23 (aa 340-349) were moderately reactive. The peptides P4, P6, P11, P13 and P15 span the beta strands C, D, G and H, parts of the CD and DG loops and the complete GH loop of the fiber knob. The main epitopes of the fiber knob of Ad15 (FK15) were represented by peptides P5 (aa 198-212), P10 (aa 223-237), P12 (aa 233-247), P13 (aa 238-252), P26 (aa 303-317), P29 (aa 318-332) and P32 (aa 333-347), spanning the beta strands B, D, G, H and I, partly strand C, the CD loop, parts of the AB and DG loops, the GH and HI loops and the N-terminal part of the IJ loop. The amino acid sequence alignment showed that the location of the linear FK8 and FK15 epitopes was found to be overlapping to a major extent. Two serotype-specific epitopes were determined on FK15, represented by P10 and P13.
Insights
This study identified linear antigenic epitopes on adenovirus fiber knobs for serotypes 8 and 15. Researchers found significant overlap in epitope locations between the two serotypes, with two specific epitopes identified on Ad15.
Area of Science:
- Virology
- Immunology
- Structural Biology
Background:
- Adenovirus fiber knobs are key targets for antibody recognition.
- Understanding linear antigenic epitopes is crucial for vaccine and diagnostic development.
- Subgenus D adenoviruses, including serotypes 8 and 15, are clinically relevant.
Purpose of the Study:
- To map the linear antigenic epitopes on the fiber knobs of adenovirus serotypes 8 (Ad8) and 15 (Ad15).
- To investigate the binding of antibodies to overlapping peptides covering the fiber knob regions.
- To identify potential serotype-specific epitopes for diagnostic or therapeutic applications.
Main Methods:
- Utilized polyclonal virus- and fiber-specific rabbit antibodies.
- Tested antibody binding to overlapping peptides spanning the fiber knob of Ad8 (FK8) and Ad15 (FK15).
- Analyzed peptide reactivity to determine main and moderate antigenic epitopes.
Main Results:
- Identified main linear antigenic epitopes on FK8 (peptides P4, P6, P11, P13, P15) and FK15 (peptides P5, P10, P12, P13, P26, P29, P32).
- Observed significant overlap in the location of linear epitopes between FK8 and FK15.
- Determined two serotype-specific epitopes on FK15 (peptides P10 and P13).
Conclusions:
- The study successfully mapped linear antigenic epitopes on Ad8 and Ad15 fiber knobs.
- The extensive overlap suggests conserved antigenic regions, while specific epitopes offer potential for serotype differentiation.
- Findings contribute to the understanding of adenovirus-host interactions and inform future immunotherapeutic strategies.