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[Effect of hydrogen peroxide on expression of interleukin-2 gene in mouse splenocytes]
The effect of hydrogen peroxide on interleukin-2(IL-2) production and IL-2 mRNA expression in mouse splenocytes was detected by cellular immunological and molecular biological methods. The results were that the level of IL-2 activity was significantly elevated in 10(-4) mol.L-1 of H2O2 as compared with the control group (P < 0.01), was obviously inhibited in 10(-3)-10(-2) mol.L-1 of H2O2(P < 0.01), and had no effect in 10(-8)-10(-5) mol.L-1 of H2O2. The results of dot blotting were that 10(-4) mol.L-1 of H2O2 enhanced IL-2 mRNA expression, while 10(-3) mol.L-1 inhibited it, and 10(-5) mol.L-1 had no effect in mouse splenocytes. These results suggest that the effect of H2O2 on IL-2 production may be mediated by regulating IL-2 mRNA expression in mouse splenocytes.
The effect of hydrogen peroxide on interleukin-2(IL-2) production and IL-2 mRNA expression in mouse splenocytes was detected by cellular immunological and molecular biological methods. The results were that the level of IL-2 activity was significantly elevated in 10(-4) mol.L-1 of H2O2 as compared with the control group (P < 0.01), was obviously inhibited in 10(-3)-10(-2) mol.L-1 of H2O2(P < 0.01), and had no effect in 10(-8)-10(-5) mol.L-1 of H2O2. The results of dot blotting were that 10(-4) mol.L-1 of H2O2 enhanced IL-2 mRNA expression, while 10(-3) mol.L-1 inhibited it, and 10(-5) mol.L-1 had no effect in mouse splenocytes. These results suggest that the effect of H2O2 on IL-2 production may be mediated by regulating IL-2 mRNA expression in mouse splenocytes.