[Determination of chloroquine in human urine]

A GC method was established for qualitative and quantitative determination of chloroquine in human urine. The chloroquine is extracted from 2 ml urine with 2 x 2 ml cyclohexame: chloroform (8:2). The retention time of chlorquine is 9.44 min. The linear of quantitation is 0-50 micrograms/ml. The recovery of the procedure in 25 micrograms g/ml is 87.0%, CV = 7. 9% (n = 5). The limit of the determination is 200 ng/ml. The method can also be used in blood. A case is reported here. In that, we detected chloroquine in urine and blood. The quantitation of chloroquine in urine and blood is 0.475 mg/ml and 3.68 micrograms/ml, respectively. A metabolite of chloroquine-desethylchloroquine is also found in the urine.