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Updated: Aug 4, 2026

Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface
Published on: February 21, 2011
[Study on induced mutagenesis interaction of the high temperature and/or cigarette smoke]
Abstract:
The study aim is to research the genotoxicity and interaction of the High temperature and/or cigarette smoke. The micronucleus of human lymphocytes and bone marrow in mice were observed following heat stress and/or watersoluble cigarette smoke substances. The results indicated that the high temperature (40 degrees C, 41 degrees C and 42 degrees C for 5 min, respectively) significantly induced micronuclei in human lymphocytes in vitro (P < 0.01). A significant synergistic effect of the high temperature and cigarette was found. When high temperature combining with high concentration cigarette smoke (1.0-2.0 cigarette/20 g/d, for 10 d) was used, a significant induction and interaction (P < 0.01) on micronucleus of the bone marrow were also observed. The results indicated that high temperature substanance aberrations would be enhanced when the high temperature combines with cigarette smoke.
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