Related Experiment Video
Updated: Sep 6, 2026

Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
Published on: March 28, 2017
[Metabolic transformation of (-)-clausenamide in rat liver microsomes]
1Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050.
Abstract:
The metabolic transformation of (-)-clausenamide, isolated from the leaves of Clausena lansium (Lour.) Skeels, was studied in vitro with phenobarbital-induced rat liver microsomal incubate containing the NADPH-generating system. The constitution of the incubation system was optimized and a RP-HPLC-DAD method was developed for the on-line analysis of (-)-clausenamide and its metabolites. The major metabolites were isolated and purified by silica gel column chromatography, preparative TLC and HPLC and their structures were determined as 6-OH clausenamide (CM1) and 5-OH clausenamide (CM2) by 1HNMR and MS.
More Related Videos
09:33Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by 32P-postlabeling
Published on: March 20, 2018
08:59An Intestine/Liver Microphysiological System for Drug Pharmacokinetic and Toxicological Assessment
Published on: December 3, 2020
Related Concept Videos
Drug Biotransformation: Overview
Drug Metabolism: Phase I Reactions
Drug Metabolism: Phase II Reactions
Drug Biotransformation: Overview
Pharmacokinetics in Pediatric Patients: Drug Metabolism
Pharmacogenetics of Phase II Enzymes: N-acetyltransferase, Thiopurine S-methyltransferase, UDP-glucuronosyltransferase