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Expression and effect of basic fibroblast growth factor on human cataract lens epithelial cells
Xingchao Shentu1, Ke Yao, Chaohui Sun
1Institute of Ophthalmology, Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China.
Objective:
To detect the expression of basic fibroblast growth factor (bFGF) in human ocular tissues, and to assess the effect of bFGF on the proliferation of human cataract lens epithelial cells (LECs) and its correlation with age.
Methods:
Enucleated eyes were subjected to immunostaining for bFGF protein. Human cataract LECs were cultured in vitro, and treated with bFGF for 48 hr. Proliferation was estimated by the positive area ratio of proliferating cell nuclear antigen (PCNA) in immunohistochemistry.
Results:
bFGF protein was found in various human ocular tissues. bFGF stimulated human cataract LEC proliferation, and there was an age-related decrease in responsiveness of human cataract LECs to bFGF (P < 0.05).
Conclusion:
bFGF might play an important role in the proliferation of residual human cataract LECs after cataract surgery.