Related Experiment Video
Updated: Oct 1, 2026

Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury
Published on: March 7, 2022
Association of the phosphatidylinositol signal pathway with prolonged myocardial ischemia
Xiuyun Ding1, Jiachang Yue, Shiwen Wang
1Institute of Geriatric Cardiology, General Hospital of PLA, Beijing 100853, China. Dingxiuyun@mailexcite.com
Objective:
To study the changes in activity of phosphatidylinositol 4 kinase (PI 4 kinase), phosphatidylinositol 4 phosphate 5 kinase (PIP 5 kinase) and protein kinase C (PKC) during myocardial ischemia and elucidate the relationship between phosphatidylinositol signal pathways and prolonged myocardial ischemia.
Methods:
In vivo an ischemic rat model was used. Activity of PI 4 kinase, PIP 5 kinase and PKC were measured at different times in postischemic heart cells using isotope analysis.
Results:
The activity of PI kinase, PIP kinase and PKC in the myocardium increased to peak at 1 hour postischemia, with activities 6.1, 3.0 and 4.0 fold over control levels, respectively. Their activities declined to normal levels with time.
Conclusion:
The phosphatidylinositol signal pathway is involved in prolonged myocardial ischemia, but its mechanism needs further study.
Related Concept Videos
Myocarditis I: Introduction
Phosphoinositides and PIPs
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
IP3/DAG Signaling Pathway
Amplifying Signals via Second Messengers

