Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cholangiocytes exhibit dynamic, actin-dependent apical membrane turnover.

R Brian Doctor1, Rolf Dahl, Laura Fouassier

  • 1Division of Gastroenterology/Hepatology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA. brian.doctor@uchsc.edu

American Journal of Physiology. Cell Physiology
|April 10, 2002
PubMed
Summary

Cholangiocytes exhibit high rates of membrane trafficking, involving subapical vesicles and actin cytoskeleton. Cyclic AMP (cAMP) and actin dynamics significantly influence this vesicular transport process.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibition of the ZEB1-BCL2 axis in cancer-associated fibroblasts as a strategy to improve chemotherapy response in intrahepatic cholangiocarcinoma.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

A subset of hepatic fibroblasts marked by Gli1 expression generates portal fibrosis and promotes ductular reaction.

JHEP reports : innovation in hepatology·2026
Same author

Epithelial to Mesenchymal Transition Transcriptional Regulator ZEB1 in Liver Cancer: Oncogenic Roles and Therapeutic Potential.

International journal of molecular sciences·2025
Same author

Loss of Aquaporin-1 in Tumor Cells Fosters Intrahepatic Cholangiocarcinoma Progression.

The American journal of pathology·2025
Same author

Laser-activated nanoparticles rewire tumor microenvironment enhancing PD-1 blockade and T cell response in cholangiocarcinoma.

Hepatology (Baltimore, Md.)·2025
Same author

NADPH oxidase 1/4 dual inhibition impairs transforming growth factor-beta protumorigenic effects in cholangiocarcinoma cancer-associated fibroblasts.

Signal transduction and targeted therapy·2025

Area of Science:

  • Cell Biology
  • Membrane Biology
  • Bile Duct Physiology

Background:

  • Cholangiocytes, the epithelial cells lining the bile ducts, play a crucial role in bile secretion and modification.
  • Understanding membrane trafficking in cholangiocytes is essential for comprehending bile duct function and disease.

Purpose of the Study:

  • To investigate the rates and mechanisms of vesicular trafficking at the apical membrane of cholangiocytes.
  • To determine the role of the actin cytoskeleton and cyclic AMP (cAMP) signaling in cholangiocyte membrane dynamics.

Main Methods:

  • Histological, biochemical, and electrophysiological techniques were employed.
  • FM 1-43 fluorescence was used to quantify exocytosis and endocytosis rates.
  • Phalloidin staining assessed actin cytoskeleton involvement; cytochalasin D and jasplakinolide disrupted actin.

Related Experiment Videos

Main Results:

  • Significant basal rates of exocytosis (1.33%/min) and endocytosis were measured in cholangiocytes.
  • cAMP analog (CPT-cAMP) increased exocytosis by 71%, while a protein kinase A inhibitor (Rp-cAMPS) decreased it by 53%.
  • Actin cytoskeleton disruption with cytochalasin D or jasplakinolide inhibited membrane trafficking.

Conclusions:

  • Cholangiocytes exhibit a high rate of apical membrane vesicular trafficking.
  • Both cAMP signaling and the actin cytoskeleton are critical regulators of membrane trafficking in cholangiocytes.
  • Subapical vesicles, partly derived from endocytosis, are integral to this process.