Related Experiment Video

Updated: Jan 21, 2026

Dynamic Adhesion Assay for the Functional Analysis of Anti-adhesion Therapies in Inflammatory Bowel Disease

Dynamic Adhesion Assay for the Functional Analysis of Anti-adhesion Therapies in Inflammatory Bowel Disease

Published on: September 20, 2018

Leukotriene D4 upregulates eosinophil adhesion via the cysteinyl leukotriene 1 receptor

Makoto Nagata¹, Keiko Saito, Koji Tsuchiya

¹Pulmonary Division, Second Department of Internal Medicine, Saitama Medical School.

The Journal of Allergy and Clinical Immunology

|April 10, 2002

Abstract

Background:

Eosinophils (EOS) are one of the cellular sources of cysteinyl leukotrienes (cysLTs) in allergic inflammation. There is evidence that cysLT(1)receptor antagonists possess anti-inflammatory properties in vivo in asthmatic airways. Although the exact mechanism of action remains unknown, cysLTs might regulate the cellular responses involved in allergic inflammation.

Objective:

The present study was undertaken to examine whether LTD(4)modifies the adhesive property of EOS.

Methods:

EOS were isolated from the blood of healthy subjects. Their adhesion to tissue culture plates or recombinant human (rh) adhesion proteins was then examined in the presence or absence of LTD(4).

Results:

LTD(4)significantly augmented EOS adhesion to tissue culture plates (adhesion: 5.0% +/- 0.5% by medium control vs 9.1% +/- 1.2% by 1 micromol/L; P <.01; n = 10). The enhanced adhesion induced by LTD(4) was blocked by pranlukast, a cysLT(1) receptor antagonist, or an anti-beta(2) integrin antibody. Flow cytometry analysis revealed that LTD(4) significantly enhanced the expression of CD11b and CD18 on the EOS surface. Finally, LTD(4) augmented EOS adhesion to rh intercellular cell adhesion molecule 1 but not to rh vascular cell adhesion molecule 1 or fibronectin.

Conclusions:

The results suggest that LTD(4) directly upregulates the adhesive property of EOS via the cysLT(1) receptor and beta(2) integrin. LTD(4) generated from EOS or cells of some other type might contribute to the development of phenotypic change in airway EOS.

More Related Videos

Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin

Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin

Published on: December 23, 2010

Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure

Bioluminescence Imaging of Heme Oxygenase-1 Upregulation in the Gua Sha Procedure

Published on: August 28, 2009

Related Concept Videos

Antiasthma Drugs: Leukotriene Modifiers

Antiasthma Drugs: Leukotriene Modifiers

Leukotriene modifiers, or cysteinyl leukotriene receptor antagonists, are medications used to manage chronic asthma. These agents target specific inflammatory mediators produced during arachidonic acid metabolism, an essential process in generating inflammation in the body.
Leukotriene modifiers work through two distinct mechanisms:

Allergic Reactions

Allergic Reactions

Adhesion

Adhesion

Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules.
Capillary action is a result of water’s adhesive tendencies. When a narrow...

Chemotherapy-Induced Nausea and Vomiting: Neurokinin-1 Receptor Antagonists

Chemotherapy-Induced Nausea and Vomiting: Neurokinin-1 Receptor Antagonists

Neurokinin 1 (NK1) receptors are distributed across the GI tract, vagal afferents, and key CNS regions including the central vomiting center and chemoreceptor trigger zone (CTZ) Chemotherapy agents stimulate enterochromaffin cells in the gastrointestinal (GI) tract to release large amounts of substance P (SP). SP is a neuropeptide released by specific sensory nerves in response to many different stressors, including those in the GI mucosa affected by chemotherapy. SP binds and activates...

Internal Receptors

Internal Receptors

Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.

Receptor-mediated Endocytosis

Receptor-mediated Endocytosis

Articles linked to this work by shared authors, journal, and citation graph.

Salivary alpha-amylase as a biomarker for obstructive sleep apnea severity and continuous positive airway pressure adherence: A cluster-based phenotyping approach.

Respiratory investigation·2026

Polymorphic tail of HLA-DQ leads to the heterogeneous IL-10 response of antigen-presenting cells.

International immunology·2026

A case of pulmonary Mycobacterium kansasii presenting with an atypical extensive consolidation involving the entire right lower lobe.

Respiratory medicine case reports·2026

Drug delivery system (DDS)-inspired cosmetic liposomes: Unveiling physicochemical determinants of skin penetration under practical application conditions.

Colloids and surfaces. B, Biointerfaces·2026

Real-World Efficacy of Ustekinumab versus Vedolizumab for Ulcerative Colitis: Differences between Short- and Long-Term Outcomes Based on Endoscopic Remission.

Inflammatory intestinal diseases·2026

Impact of Bowel Rest on Hospitalized Patients with Ulcerative Colitis.

Inflammatory intestinal diseases·2026

See all related articles