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Volume-activated chloride current in pigmented ciliary epithelial cells.

L X Chen1, L W Wang

  • 1Department of Physiology, Guangdong Medical College, Zhanjiang 524023, China.CHENL1@cardiff.ac.uk

Sheng Li Xue Bao : [Acta Physiologica Sinica]
|April 10, 2002
PubMed
Summary

A volume-activated chloride current in bovine pigmented ciliary epithelial cells was characterized. This current, dependent on intracellular ATP, shows outward rectification and may involve P-glycoprotein.

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Area of Science:

  • Cell Physiology
  • Ion Transport
  • Ocular Biology

Background:

  • Pigmented ciliary epithelial (PCE) cells play a crucial role in ocular fluid dynamics.
  • Understanding ion transport mechanisms in PCE cells is vital for ocular health.

Purpose of the Study:

  • To characterize the volume-activated chloride current in bovine PCE cells.
  • To investigate the properties and potential molecular basis of this current.

Main Methods:

  • Whole-cell patch clamp technique was used to record currents in response to hypotonic challenge.
  • Pharmacological agents (ATP, tamoxifen) were applied to assess current modulation.
  • Current-voltage relationships were analyzed to determine reversal potentials.

Main Results:

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  • Hypotonic exposure induced a volume-activated chloride current dependent on intracellular ATP.
  • The current exhibited outward rectification and minimal time-dependent inactivation.
  • Extracellular ATP and tamoxifen significantly inhibited the current, suggesting a role for P-glycoprotein.

Conclusions:

  • A distinct volume-activated chloride current exists in bovine PCE cells.
  • This current shares properties with P-glycoprotein-associated currents, implicating P-glycoprotein in its activation.
  • Further research into this current's role in ocular physiology is warranted.