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The molecular characterization of maize B chromosome specific AFLPs
Zhong Xia Qi1, Hui Zeng, Xiu Lan Li
1The College of Life Sciences, Nankai University, Tianjin, China.
Cell Research
|April 11, 2002
Abstract:
The origin and evolution of B chromosomes could be explained by the specific DNA sequence on them. But the specific sequences known were quite limited. To investigate maize B chromosome sqicific DNA sequeces, maize genomes with and without B chromosomes were analyzed by AFLP. Only 5 markers were found specific to genomes with B chromosomes among about 2000 AFLP markers. Southern hybridization and sequence analysis revealed that only the sequence of M8-2D was a B chromosome specific sequence. This sequence contained the telomeric repeat unit AGG