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An in vitro screening method for DNA cytosine-C5-methylase inhibitor
Takashi Tamura1, Atsushi Kataoka, Li Yin Shu
1Department of Bioresources Chemistry, Faculty of Agriculture, Okayama University, Japan. tktamura@cc.okayama-u.ac.jp
Natural Product Letters
|April 11, 2002
Abstract:
A specific inhibitor of DNA cytosine C5-methylases would be useful for studying genomic imprinting, X-chromosome inactivation, carcinogenesis, and regulation of tissue-specific gene expression, for these physiological phenomena appears to be regulated through DNA methylation in promoter sequences. This paper reports a novel convenient in vitro assay method for screening DNA cytosine C5-methylase inhibitor. Our method uses a commercially available Hae III methylase (cytosine C5 methylase), its corresponding Hae III endonuclease, and lambda DNA as their substrate.