Electro-orientation of Schizosaccharomyces pombe in high conductivity media

The orientation of microbial cells may be important in cell-cell interactions within microbial consortia. As part of our research programme aimed at the construction of Artificial Structured Microbial Consortia (ASMC), we have investigated the electro-orientation of Schizosaccharomyces pombe in AC electric fields, and studied the effects of the applied frequency, voltage, and distance between the electrodes, at different medium conductivities. It is shown that the electro-orientation of S. pombe in media with conductivities similar to that of growth media is feasible using microelectrodes. Oriented growth of S. pombe can be obtained when continuously exposed to AC electric fields in growth medium over extended periods.