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A filtration, incubation and staining reactor including a new protocol for FISH
Lothar Poschen1, Peter Klauth, Joost Groeneweg
1Forschungszentrum Jülich, Institut für Chemie und Dynamik der Geosphäre (ICG), Institut IV: Agrosphäre, Germany. l.poschen@fz-juelich.de
Journal of Microbiological Methods
|April 12, 2002
Abstract:
A newly developed device for performing fluorescence in situ hybridization (FISH) is described. An adapted procedure was compared with two typical FISH protocols. Tests were performed with Pseudomonas cells and the gene probe EUB338. With the novel procedure, we obtained a better recovery of cells and less variability in results.