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Updated: Oct 1, 2026

Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case Study
Published on: January 31, 2014
[Effect of bacterial physiological state and heavy metals on the hyperactive reaction of tobacco leaf]
R I Gvozdiak1, L A Pasichnyk, S F Khodos
1Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Abstract:
It has been shown that the initiation and proceeding of hypersensitive reaction on the tobacco leaf under the bacterial effect are influenced by the infectious loading of the pathogen, physiological state of bacteria, their viability, temperature of the environment and heavy metal salts. It has been established that the hypersensitive reaction is most distinctly manifested when 1-3 days bacterium cultures are used. Their activity decreases essentially with age, and 10-14 days old cultures are not able to evoke the hypersensitive reaction. The bacterial cells being inactivated with temperature and UV-irradiation lose the ability to evoke the hypersensitive reaction. The reaction of hypersensitive is inhibited by salts of heavy metals in concentrations 0.1-1% and by high temperature of the environment (30 degrees C).
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