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1H detected (13)C echo planar imaging.

Alex M J Hudson1, Walter Köckenberger, Michael Heidenreich

  • 1Magnetic Resonance Centre, School of Physics and Astronomy, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|April 12, 2002
PubMed
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Cyclic J cross polarisation (CYCLCROP) with echo planar imaging (EPI) offers enhanced (13)C imaging. This method improves temporal resolution for studying metabolic transport and accumulation in plants.

Area of Science:

  • Magnetic Resonance Imaging
  • Biophysics
  • Plant Physiology

Background:

  • Noninvasive monitoring of (13)C distributions and fluxes is crucial for understanding metabolic processes.
  • Existing methods face challenges with sensitivity and RF power deposition.
  • Improving temporal resolution in (13)C imaging is essential for dynamic studies.

Purpose of the Study:

  • To demonstrate the feasibility of echo planar proton detected (13)C imaging using CYCLCROP with the PRAWN module.
  • To apply this technique for measuring (13)C-labelled sucrose transport and accumulation in castor bean seedlings.
  • To present methods for eliminating J-splitting effects and a fast B(1) field-mapping technique.

Main Methods:

  • Utilized Cyclic J cross polarisation (CYCLCROP) with the PRAWN rotating frame Hartmann-Hahn mixing sequence.

Related Experiment Videos

  • Integrated CYCLCROP with echo planar imaging (EPI) for spatial encoding of proton-detected carbon signals.
  • Developed methods to eliminate J-splitting in echo planar images and a fast B(1) field-mapping technique.

    • Main Results:

    • Demonstrated the feasibility of echo planar proton detected (13)C imaging using CYCLCROP-PRAWN.
    • Successfully applied the technique to measure (13)C-labelled sucrose transport and accumulation in castor bean seedlings.
    • Presented effective methods for J-splitting removal and a rapid, quantitative B(1) field-mapping method.

    Conclusions:

    • The CYCLCROP-EPI technique significantly improves temporal resolution for (13)C imaging.
    • This method enables efficient, noninvasive studies of metabolic transport and accumulation.
    • The developed B(1) field-mapping is fast and quantitative, valuable for dual-resonant probes.