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M Ellenberger1, L Brehamet, M Villemin

  • 1Département de Biologie - Centre d'Etudes Nucléaires de Saclay B.P. no. 2-91-GIF-sur, France

FEBS Letters
|June 1, 1970
PubMed
Summary
This summary is machine-generated.

This study reveals how the spin-spin coupling constants of adenosine diphosphate (ADP) and adenosine triphosphate (ATP) change with pH. These findings indicate structural differences in the triphosphate chain of nucleotides.

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Area of Science:

  • Biochemistry
  • Chemical Physics
  • Nuclear Magnetic Resonance Spectroscopy

Background:

  • Adenosine diphosphate (ADP) and adenosine triphosphate (ATP) are crucial molecules in cellular energy transfer.
  • Understanding the structural and chemical properties of ADP and ATP is vital for comprehending biological processes.

Purpose of the Study:

  • To investigate the pH dependence of spin-spin coupling constants J (P-O-P) in ADP and ATP.
  • To determine the pK values associated with these molecules.
  • To compare the structural characteristics of ADP and ATP with tripolyphosphate.

Main Methods:

  • Phosphorus Nuclear Magnetic Resonance (31P NMR) spectroscopy was employed.
  • Measurements were conducted at a controlled temperature of 0 degrees Celsius.

Related Experiment Videos

  • NMR spectral data were analyzed to determine pH-dependent variations in coupling constants.

    • Main Results:

    • The pH dependence of J (P-O-P) coupling constants for ADP and ATP was successfully mapped.
    • Analysis of the variation curves as titration curves yielded distinct pK values for ADP and ATP.
    • A comparison with tripolyphosphate suggested the presence of structural deformation within the triphosphate chain of these nucleotides.

    Conclusions:

    • The study provides insights into the protonation states and structural dynamics of ADP and ATP through NMR spectroscopy.
    • The determined pK values offer a quantitative measure of the ionization behavior of specific phosphate linkages.
    • Evidence of structural deformation in the triphosphate chain highlights the conformational flexibility of these essential biomolecules.