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Purification of soluble murine transplantation antigens by isoelectric focusing

P Jolles1, F Schoentgen, O Halle-Pannenko

  • 1Laboratory of Biochemistry, Faculty of Science, 96 Bd. Raspail, 6o, Paris, France

FEBS Letters
|June 1, 1970
PubMed

Hydrosoluble transplantation antigens were prepared from membranes and microsomes of C(3)H mice (BP8 tumoral cells) and purified by isoelectric focusing. A biologically active fraction which seems homogenous by acrylamide gel electrophoresis was characterized: it specifically inhibits anti C(3)H hemagglutinin antibodies and provokes a highly significant prolongation of skin graft.

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